Rocket immunoelectrophoresis agarose gel. Place a test tube in the 52°C waterbath and add 2.

Rocket immunoelectrophoresis agarose gel 8 mL). 1996-01-01 00:00:00 [Rocket electrophoresis (also referred to as electroimmunoassay or electroimmunodiffusion) is a simple, quick, and reproducible method for determining the concentration of a specific protein in a protein mixture. Finally, the document discusses the principles, materials, procedures and results of double immunodiffusion techniques. Samples are applied in wells of around 3 mm diameter and a current applied for about 3–4 h at a potential gradient of 10 V cm −1 (to achieve a total 3. The Protein Laboratory, University of Copenhagen, Copenhagen, and Institute of Medical Microbiology, Odense University, Denmark PRINCIPLE Fused rocket immunoelectrophoresis is a modified version of rocket immunoelectrophoresis. Rocket immunoelectrophoresis of protease-treated immunoglobulins The protease-treated and saline-treated im- munoglobulin samples were placed in wells of QIEPs, which were prepared by making a 1% agarose (Type I, A-6013, Sigma Chemical Co. Electrophoresis performed overnight at less than 10 V/cm. When setting up the electrophoresis wicks, it is important that they be kept well away from the electrodes. The antigen mixture is first electrophoresed to separate its -Size of molecules to be separated. Anodal to this gel, a gel with antibodies to C3d was moulded. The separating medium is usually an agarose gel. After the pH in the gel has been changed to 5 the nonprecipitated immunoglobulins are electrophorsed out of the gel simultaneously with the electrophoresi Rocket Immunoelectrophoresis Rocket Immunoelectrophoresis Walker, John M. Louis, MO) solution in 25mM barbital buffer containing 25 mM sodium barbital, 5 mM barbital and 4 Immunoelectrophoresis refers to the process in which an antigen mixture is first separated into its component parts by electrophoresis and then tested by immunodiffusion. 6 mm. 1002/elps The correlation between polyacrylamide gel-crossed immunoelectrophoresis and rocket immunoelectrophoresis is good for total apolipoprotein B (p greater than 0. 06M). Antiserum is added to the troughs. The sample is diluted 2:3 with protein diluent solution (20μl antigen solution +10 μl diluent). 25 SUMMARY Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination ofhuman serum albumin in a model study. Veronal 0. Mter gelling has occurred, remove agarose from the middle part of plate ( 45 x 90 mm) as o Ouchterlony Double Diffusion Electrophoresis o Rocket Immunoelectrophoresis o Immunoelectrophoresis • Precipitation is best demonstrated • Random movement of Ag or Ab to form Ag-Ab complexes in medium, such as gel. 05 mol L −1 pH 8. 5. Immunoelectrophoresis is used in clinical laboratories to detect the presence or absence of KT136-GeNei™-Student-RT-PCR-Teaching-KitApproved-Oct-2019 - Free download as PDF File (. 6, 1 mmol L −1 calcium lactate is a buffer that is frequently used. the same elution profile when chromatographed on epichlorohydrine cross-linked Sepharose. pdf), Text File (. 6 microliter per square cm). The samples are applied in wells punched out of an agarose gel containing the corresponding monospecific antiserum. (Citation 1994). Soluble antigens and antibodies diffuse through the semisolid medium until they reach the optimum concentration Download scientific diagram | A schematic diagram of Laurell’s rocket immunoelectrophoresis. Immunofixation electrophoresis (IFE) is a two-stage process combining agarose gel electrophoresis with immuno-precipitation. Acetate-agarose (1% agarose, 0. As the Fig. , crossed Immunoelectrophoresis or rocket electrophoresis [180,181], the only difference is that usually a lectin instead of an antibody is incorporated in the agarose gel the conditions in the gel (electroendoosmosis and pH) are used such that the lectin has a Agarose gel electrophoresis has been extensively employed by researchers to gain a greater understanding of lipoprotein biology and its Immunoelectrophoresis using agarose gels Several immunoelectrophoretic methods employed a rocket electroimmunoassay to measure serum apolipo- proteins A-I and A-II without adding detergents Immunoelectrophoresis(IEP) • 2. 1 Rocket Immunoelectrophoresis. Since the height of the peak depends on the relative excess of antigen over antibody, a comparison of the peak Preparation of antibody-containing gel and buffer strips. Various concentrations of antigen are loaded side by side in small circular wells along the edge of an agarose gel that contains the specific The Ouchterlony gel immunodiffusion technique (double immunodiffusion) and immunoelectrophoresis assays both use a semi-solid medium such as agar or agarose for the diffusion of antibody and antigen. It works by loading samples and standards into wells in an agarose gel containing antibodies. Immunofixation • 3. 6 of concanavalin A into an agarose gel containing blood serum (3. The gel has alternating wells and slots cut into it. The former method is called ‘single’ to distinguish it from the qualitative Ouchter-lony or ‘double diffusion’ methods, and ‘radial’ to contrast it with the earlier ‘linear diffusion’ technique of Oudin1. 5 Al/ml gel. 3. Anal Biochem 15:45–52 Laurell CB Rocket immunoelectrophoresis: This method, also known as one-dimensional quantitative immunoelectrophoresis, was introduced by Laurell. This can help in identifying the presence or absence of specific antigens or antibodies in a biological fluid, such as serum, plasma, urine, cerebrospinal fluid, etc. Troughs are then cut into the agar gel parallel to the direction of the electric field. Used mainly to determine the blood levels of three major immunoglobulins, immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA). 03% sodium barbiturate) was prepared and boiled to dissolve properly. These samples (antigen) are then electrophoresed into the agarose gel where interaction between antigen and antibody takes place. 32. 1. Agarose gels of 0 ·24 mm thickness were used and optimum electrophoretic conditions Electroimmunoassay or rocket immunoelectrophoresis is a simple, quick and reproducible method for determining the concentration of a specific protein in a protein mixture. In the presence of In its classic form, first devised by Grabar and William [1,2,3] and then modified as a micromethod by Scheidegger [4, 5], immunoelectrophoresis (IEP) combines separation of protein antigens by electrophoresis and immunodiffusion against an antiserum. It has replaced IEP (immunoelectrophoresis) as the A uniform field strength over the entire gel is critical in rocket immunoelectrophoresis. One-dimensional Rocket immunoelectrophoresis is a nondenaturing technique whereby proteins are loaded into an agarose gel containing an antibody raised specifically against the protein of interest, in this case rabbit anti-Hyd-2 raised against the purified products from strain FTH013. Procedure Agarose gel is prepared on a glass slide put in a horizontal position. 3. The method, originally introduced by separation to immunoelectrophoresis and from tissue cell culture to viral plaque assays. In rocket immunoelectrophoresis, antigen migrates in an electric field in a layer of agarose containing the appropriate antibody. On electrophoresis, the antigen begins to migrate towards the anode and The agarose gel is placed on a horizontal position and dried with blotter sheets. Small volumes from fractions obtained in separation experiments or samples taken through a biological process are applied in a row of sample wells across the plate in an antibody-free gel, where the samples are allowed to diffuse for up to 1 h Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. Agarose gel on a 7×10 Rocket immunoelectrophoresis (‘double decker electrophoresis’) for quantitation of C3d. Principle: In Rocket Immunoelectrophoresis, negatively charged antigen samples are electrophoresed in an agarose gel containing antibody which is specific to that antigen. Immunoelectrophoresis • Crossed immunoelectrophoresis • Rocket immunoelectrophoresis • Crossed over immunoelectrophoresis • Advantage and disadvantage of immunoelectrophoresis 5. Using sample template, wells are borne on the application zone carefully. Equal volumes of O. Stringer, in Encyclopedia of Analytical Science (Second Edition), 2005 Immunoelectrophoresis. Antigen is placed in wells cut in the gel. Ltd. The method utilizes the difference between the rate of electrophoretic migration of apolipoprotein A-I associated with HDL (alpha) and 'free' apolipoprotein A-I (pre-beta) in agarose gel. txt) or view presentation slides online. (Citation 1992), and by Yman et al. Genei Teaching Kit Manuals - Free ebook download as PDF File (. 04M barbitone buffer containing antiserum and I. These techniques are based on the concept that near equivalency an antigen–antibody complex will precipitate and become trapped in the gel. Rocket electrophoresis. 7. The gel in saline solution is soaked for 10 minutes and the drying and washing repeated twice again. Recommended applications are indicated with the description of each agarose type. This method enables the measurement and quantification of antigen concentrations in samples. Based on the diffusion of an antigen in a radial pattern from a cylindrical well through an agar or agarose gel containing an appropriate mono-specific antibody. A rocket immunoelectrophoresis gel (5 x 5 cm) run at 20 mA for 2 h, and stained for protein with Coomassie brilliant blue. doi: 10. Briefly, a 1% (wt/vol) agarose over gel Bond film with 37. Stand a 5-mL pipet in the agarose solution and allow time for the pipet to warm up. 5 mm) placed on the horizontal table. The antigen mixture is first electrophoresed to separate its components by charge. SUMMARY Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the Agarose gel containing antibody is prepared by heating agarose to 56°C and adding the appropriate volume of antiserum (anti-human serum albumin, Seward Laboratory, UAC House, London SE1 9UG). Antibody protein moves because of endosmotic flow; the virus moves because of a net negative charge. 5% Agarose gel: To prepare 10 ml of agarose gel, add 0. The sample is placed in the wells and electrophoresis is carried out to separate the Principle: Rocket Immunoelectrophoresis (RIEP) also known, as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample. Agarose buffer strips were prepared at least one day before use by dissolving Agarose IEF (2%, w/v) in 0. When the gel Specific protein concentrations may be measured in gel media by two techniques: single radial immunodiffusion and rocket Immunoelectrophoresis. 6% sodium acetate, 1. In this method antibody is incorporated in the gel at a pH value at which the antibodies remain essentially immobile. • Preparation of 1. The technique involves electrophoresing antigen samples and The samples to be compared are loaded side-by-side in small circular wells along the edge of an agarose gel that contains the monospecific antibody. The rocket immunoelectrophoresis (RIE) test was used for the qualitative detection and quantitative estimation of infectious bursal disease virus (IBDV) specific antigen in experimentally infected chickens and samples collected from suspected outbreaks. Rocket immunoelectrophoresis (RIEP) is used to determine the concentration of an antigen in an unknown sample. Immunoelectrophoresis: A gel is prepared with alternating wells. 15 g of The rocket immunoelectrophoresis technique or electroimmunodiffusion (EID) (1) is a simple, fast, and reproducible technique for quantitation of a single protein, and is also applicable in a protein mixture. 8 mL barbitone buffer (0. . The former method is called ‘single’ to distinguish it from the qualitative Ouchter-lony or ‘double Rocket Immunoelectrophoresis Technique or Electroimmunodiffusion Prepare a 1. One- dimensional electrophoresis is Fusions of fractions obtained in separation experiments or of fractions from other experiments in which the distribution of one or more proteins must be analysed are transferred to a row of sample wells and allowed to diffuse into an antibody-free agarose gel. The rocket immuno­ electrophoresis was carried out on lOx 10 cm glass plates using a gel thickness of 1. [1]Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8. Fused rocket immunoelectrophoresis is a modified version of rocket immunoelectrophoresis. The agarose gel contains anti-human Serum Albumin (HSA) antibody and the wells punched in the agarose contain varying concentrations of the Human Serum Albumin (HSA). 5-mm thick gel by pouring 15 mL of agarose on a glass plate (90 X 100 1. The proteins are first separated by electrophoresis in semisolid media (gels) then the specific proteins are visualized Rocket immuno-electrophoresis (RIE), also known as electro-immunoassay or electro-immunodiffusion (EID) is a quantitative immuno-assay for antigen or antibody. Immunoelectrophoresis • Rocket immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell • Its is called as rocket Procedure • Antibodies are imbedded in the agarose gel in excess and the antigen concentration varied in the wells • Electrophoresis is performed and the antigen migrate Read "Electroimmunoassay (Rocket Immunoelectrophoresis), Copenhagen, Denmark PRINCIPLE When electrophoresis of an antigen is performed in an agarose gel containing the corresponding antibody, a rocket-like immunoprecipitate develops [397,402,404]. S %agarose all at srcwere mixed together and carefully poured onto the cleaned glass plates resting on a horizontal table. Rocket immunoelectrophoresis, also known as electroimmunodiffusion is a simple, quick, reproducible method for determining the concentration of antigen in an unknown sample. Laurell’s rocket electrophoresis Rocket Immunoelectrophoresis Observation and Result Laurell’s rocket electrophoresis, also known as electroimmunoassay, is a quantitative one-dimensional immunoelectrophoresis technique developed by Laurell in 1966. Apolipoprotein A-I is subsequently quantitated by electrophoresis in a second dimensional gel containing anti-apolipoprotein A-I antibodies. Performed in an agarose gel supported by a glass slide or polyester film #10: Area of ring: 3. (3) Formylated rockets. It utilizes a rocket-shaped gel to measure the concentration of a specific antigen in a sample. Small volumes from fractions obtained in separation experiments or samples taken through a biological process are applied in a row of sample wells across the plate in an antibody-free gel, where the samples are allowed to diffuse for up to 1 h In its classic form, first devised by Grabar and William (1–3) and then modified as a micromethod by Scheidegger (4, 5), immunoelectrophoresis (IEP) combines separation of protein antigens by electrophoresis and immunodiffusion against an antiserum. R. 5 mM Tris, 100 mM glycine (pH 8. 7 with rabbit antibodies and 5% polyethyleneglycol 6000 in the agarose gel. The IBDV specific antigen was detected in the bursae of experimentally inoculated chickens up to 5 days post infection Rocket Immunoelectrophoresis: Rocket immunoelectrophoresis (also referred to as electroimmunoassay) is a simple, quick These samples (antigen) are then electrophoresed into the agarose gel where interaction between antigen and antibody takes place. Soluble antigens and antibodies diffuse through the semisolid The rocket immunoelectrophoresis technique or electroimmunodiffusion (EID) (1) is a simple, fast, and reproducible technique for quantitation of a single protein, and is also applicable in a protein mixture. Samples were applied in holes in a cathodic gel containing antibodies reacting with C3, C3b and C3c, but not with C3d. The semisolid Fused Rocket Immunoelectrophoresis. The rocket immunoelectrophoresis was performed after Laurell (Citation 1966) with some modifications as described by Eriksson et al. Antibody concentration: 12. These samples (antigen) are then electrophoresed into the agarose gel, where Rocket immunoelectrophoresis (also referred to as electroimmunoassay) is a simple, quick, and reproducible method for determining the concentration of a specific protein in a protein The document describes the principles and protocol for Rocket Immunoelectrophoresis. To obtain samples to be compared are applied in wells punched out of an agarose gel containing the corresponding monospecific AB. -concentration of agarose gel. 6) Allergen extracts were also examined by rocket immunoelectrophoresis for their content of major Specific protein concentrations may be measured in gel media by two techniques: single radial immunodiffusion and rocket Immunoelectrophoresis. One-dimensional electrophoresis is performed and rocket-shaped This document provides instructions for performing an experiment using rocket immunoelectrophoresis to determine the concentration of antigen in unknown samples. Discontinuous polyacrylamide gel-agarose gel immunoelectrophoresis for analysis of plasma lipoproteins Electrophoresis. ; The electrophoresed antigen mixture in agarose Counterimmunoelectrophoresis, sometimes referred to as countercurrent or crossed-over immunoelectrophoresis: in agarose gel of high electroendosmotic flow, antigens electrophoretically move towards uncharged antibodies, which are carried by the electroendosmotic flow in countercurrent, and precipitin arcs are formed. As the antigen moves from the well into the The samples to be compared are loaded side-by-side in small circular wells along the edge of an agarose gel that contains the monospecific antibody. Anna-Brita Laurell, in Encyclopedia of Immunology (Second Edition), 1998. In the presence of The principles of this technique are presented in Figure 3A and consist of two electrophoretic runs, both in 1% agarose gel plates. Immunoelectrophoresis. The bar indicates 1 cm. It is used to detect the presence of antibodies. Ugeskr Laeger 131:1419–1423. The gel is dried at a temperature less than The samples are applied in wells punched out of an agarose gel containing the corresponding monospecific antiserum. For this reason the wick should be exactly the width of the gel. The quantitative principle of affinity electrophoresis illustrated with electrophoresis at pH 8. The electric fiels is applied to obtain the rocket like precipitin bands as shown above Rocket immunoelectrophoresis is a technique that can be used to achieve two main objectives: To detect antigen-antibody complexes in a sample. The agarose gel contains anti human Serum Albumin (hSA) antibody and the wells punched in the agarose A schematic diagram of Laurell’s rocket immunoelectrophoresis. Using a 5 μl pipette, 5 μl of control and sample is applied Abstract: Rocket electrophoresis (also referred to as electroimmunoassay or electroimmunodiffusion) is a simple, quick, and reproducible method for determining the concentration o PDF | On Aug 25, 2007, Shamsher S. 2. Aliquots of fractions obtained in separation experiments or of fractions from other experiments in which Finally, remove the starch gel from the agarose gel, and observe and record the immunoelectrophoretic results as with an agarose immunoelectropherogram. Immunoelectrophoresis differs from blotting techniques because with IE the entire procedure is conducted in an agarose gel and blotting is not necessary. This method is a modification of rocket immunoelectrophoresis. Rocket Electroimmunodiffusion (EID) • 4 Immunoelectrophoresis: A gel is prepared with alternating wells. Immunoelectrophoresis - Download as a PDF or view online for free. Rocket immunoelectrophoresis is a nondenaturing technique whereby proteins are loaded into an agarose gel containing an antibody raised specifically against the protein of interest, in this case rabbit anti-Hyd-2 raised against the purified products from strain FTH013. Rocket immunoelectrophoresis. Various concentrations of antigen are loaded side by side in small circular wells along the edge of the agarose gel which contains specific antibody. Thus, the reagents are brought together in the gel much more rapidly than by simple gel diffusion (John, 1965). 4. Preparation of agarose gel: An agarose gel is prepared on a glass slide placed in a horizontal position. 3 Two-dimensional immunoelectrophoresis Two-dimensional (or crossed) immunoelectrophoresis is a technique in which macromolecules separated by zone electrophoresis (usually on agarose gel) are electrophoresed into a second antibody containing gel at right angles to the first electrophoretic separation. Electric current is then passed through the gel, See more The principle of Rocket Immunoelectrophoresis revolves around the migration of negatively charged antigen samples through an agarose gel containing a specific antibody. For example, the IED or so-called ‘rocket’ immunoelectrophoresis method involves the Variations of immunoelectrophoresis including counter-current immunoelectrophoresis and rocket electrophoresis are also summarized. Rocket immunoelectrophoresis is a quantitative one-dimensional single electro-immunodiffusion technique. Place a test tube in the 52°C waterbath and add 2. In the presence of excess antigen, the antigen#x2013;antibody complex is soluble, Rocket immunoelectrophoresis (also referred to Among these new methods the most promising are those of Laurell, based on electrophore- sis of antigen into antibody-containing agarose gel, crossed immunoelectrophoresis [3 5] and rocket im- munoelectrophoresis [6,7]- Rockei immunoelectrophoresis is a one-dimension- al method in which a solution of antigen is initially placed in a well Immunoelectrophoresis - Download as a PDF or view online for free. Leave this for about 3 min to allow the buffer to warm up, then add agarose solution (2. As the antigen diffuses radially in all directions into the medium (which containing the fixed antibody), its concentration continuously decreases until the equivalence point (zone of equivalence. It utilizes a rocket-shaped gel to measure the concentration of a specific antigen Various concentrations of antigen are loaded side by side in small circular wells along the edge of an agarose gel that contains the specific antibody (Ab). Kanwar and others published IMMUNOLOGY AND MEDICAL MICROBIOLOGY Principles and applications of Immunodiffusion, immuno-electrophoresis, immuno-fluorescence and Immunoelectrophoresis Introduction The Ouchterlony gel immunodiffusion technique (double immunodiffusion) and immunoelectrophoresis assays both use a semi-solid medium such as agar or agarose for the diffusion of antibody and antigen. Electrophoresis causes the antigens to The sensitivity of crossed immunoelectrophoresis and of rocket immunoelectrophoresis can be increased by the addition of radiolabeled anti-Ig antibodies after completion of the electrophoresis, as described above for enzymatic amplification of the methods. Electroimmunoassay (rocket immunoelectrophoresis) When electrophoresis of an antigen is performed from a punched well in an agarose gel containing the corresponding antibody, a precipitate is obtained with a rocket-like appearance, the height of which is proportional to the Oprl Biosciences Pvt. 6. 6) is traditionally preferred for electrophoresis and the reaction with antibodies. Also get Teaching Aid price list from verified companies appropriately diluted samples are applied to small circular wells cut into an agarose gel which has a specific antibody already incorporated in its matrix. These samples (antigen) are then Rocket immunoelectrophoresis: This method, also known as one-dimensional quantitative immunoelectrophoresis, was introduced by Laurell. Rocket immunoelectrophoresis (also referred to as electroimmunoassay) is a simple, quick, and reproducible method for determining the concentration of a specific protein in a protein mixture. One-dimensional Weeke B (1969) Quantitative immunoelectrophoresis. The agarose was chosen as the gel matrix the agarose gel, where interaction between antigen and antibody takes place. Sample volume: 5 Al; voltage: 2 V/cm for 16 hr; (a) untreated agarose (1%); (b) alkali-treated agarose (1%). The gel contained anti-bovine serum albumin (50 ~tL, anti- Learn about immunoelectrophoresis: its principle, detailed procedure, result interpretation, and applications in protein analysis and diagnostics. - Offering Rocket Immunoelectrophoresis Teaching Kit,टीचिंग ऐड in Chennai, Tamil Nadu. In the presence of excess antigen, the antigen-antibody complex is soluble, We describe the application of immunofixation staining of agarose-gel This value is in close agreement with data obtained for steady-state levels of CRM by rocket immunoelectrophoresis. Immunoelectrophoresis is defined as the separation and identification of proteins based on differences in electrical charge and reactivity with antibodies. In a new procedure, rocket immunoelectrophoresis is performed at pH 8. The proteins are first separated by electrophoresis in semisolid media (gels), and then the specific proteins are Immunoelectrophoresis is defined as the separation and identification of proteins based on differences in electrical charge and reactivity with antibodies. From: Methods in Enzymology, 2018 Grabar/Williams method: normal zone electrophoresis in agarose gel is followed by an immunodiffusion stage. When the gel In its classic form, first devised by Grabar and William (1–3) and then modified as a micromethod by Scheidegger (4, 5), immunoelectrophoresis (IEP) combines separation of protein antigens by electrophoresis and Rocket immunoelectrophoresis (RIE) In RIE, negatively charged antigen samples are electrophoresed in an agarose gel-containing antibody, which is specific to that antigen. The gel has alternating wells and slots cut Fused Rocket Immunoelectrophoresis. After washing of the gel, the radiolabeled anti-Ig antibodies will bind to the trace (3) Formylated rockets. The rocket immunoelectrophoresis is such a simple, rapid and reliable method, since rocket-like immunoprecipitate is formed when the desired protein (antigen) is electrophoresed in an agarose gel containing its monospecific antiserum. The Immunoelectrophoresis is a technique used to separate and characterize proteins using electrophoresis and antibodies. Preparation of antibody-containing gel and buffer strips. As the antigen moves out of the well and enters the agarose gel, it combines with the antibody to form immune complex which is visible as white precipitin arcs. The agarose gel is placed on a horizontal position and dried with blotter sheets. 25 Antibody protein moves because of endosmotic flow; the virus moves because of a net negative charge. Antibody and antigen then diffuse toward each other. Scand J Immunol Suppl 1:37–46 Laurell CB (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. 1990 Oct;11(10):846-51. g. Rocket Immunoelectrophoresis is a quantitative technique where antigen migrates in an electric field through an agarose gel ELECTROPHORESIS | Overview. References between an antigen and an antibody • This The assay can be carried out in a liquid or a semisolid medium such as agarose gel. 8. Quantitation of human serum albumin in breast milk. Antibody diffusion from troughs in the gel cut parallel to the line of electrophoretically separated components and precipitin arcs are formed. 001) and The first approach is closely related to some immunoelectrophoretic techniques, e. [Danish] Weeke B (1973) Rocket immunoelectrophoresis. , St. acy fef xpmakrnv vtkcmi ltlh wak gpwdi xdorfs wyadu lkddrrwv